FAQ

Faq

What cell types have you tested?: Attached cell lines: U2OS, HeLa, NIH3T3, HepG2, MCF-7, PC-12, HUVEC
Primary cells: hMSC, Mouse oocyte stem cell, PBMC (Peripheral Blood Mononuclear cell), Neuronal stem cell
Embryonic cell: Mouse embryonic cell
Does JuLI™ Stage measure cell size?: Yes, we have a software ruler and also a scale bar.
Can the JuLI™ Stage detect bacterial cells?: Yes, we can use the 20x objective lens for bacterial detection.
What kind of objective lens can we use?: JuLI™ Stage offers three different magnifications of the objective lens; 4x, 10x and 20x
How long does it take to capture 96 wells with 1 color?: It takes about 9 minutes.
What is the limit of the detection with 20x objective lens of JuLI™ Stage?: The limit of detection is 1㎛.
Can I measure the counting number of GFP/RFP cells?: Yes, we are providing the viewer software, which you can install on your desk top computer.
How do I optimize the cell migration assay for my cell type?: It depends on the assay medium and cell density, you should attempt to find out the optimal condition of your cells with trial and error.
Can I make parallel measurement of cell confluence using a fluorescent reagent?: Yes, multiplexed measurements of cell confluence can be made using GFP/RFP/DAPI imaging channel.
Is it difficult to use the stitching function in JuLI™ Stage?: It is easy. You can click the stitching toggle button on the option mode to apply stitching while monitoring. Also, you can determine the range of stitching area.
Can I use 100mm culture dishes with JuLI™ Stage?: Yes, JuLI™ Stage is compatible with microplates (6well, 24well, 48well, 96well and 384well), T25 and T75 culture flasks, Petri dishes (35mm, 60mm, 100mm).
Can I use V-Bottom well plate or clear-bottom well plate?: You can expect the better result with clear-bottom well plates. However, we do not recommend to use V-bottom well plates due to the difficulty of focusing objectives.
How come the time-lapse images become dark or bright?: * Please check the following to enhance images.
* Cell culture flask should be wetted by the culture media before making a movie.
* Warm up the instrument for 2 hours before monitoring (The power should be on when warming up the instrument).
* Eliminate any dust on the culture dish.
* Remove any condensation on the lid of the culture dish.
* To prevent any problem such as shaking of culture dish or inflowing of light, take extreme caution when you open or close an incubator door during monitoring.
If I want to change the objective lens while I am monitoring, how can I change it?: You can change the objective lens manually.
Doesn’t it have any problem caused by humidity inside an incubator such as the image is out of focus?: In order to prevent such problem, we strongly recommend the following two things before start of monitoring.
* Warm up the JuLI™ Stage for 2 hours inside the incubator.
* Coat the well plate cover with the cell culture media or the clean PBS.
What are the dimensions of the JuLI™ Stage?: It is 429 (W) X 310 (D) X 324 (H) mm. You can place it inside most incubators in your lab.
What do you chemical recommend to wipe the surface of JuLI™ Stage?: We recommend you to use 70% Ethanol.

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