What cell types have you tested?
Attached cell lines: U2OS, HeLa, NIH3T3, HepG2, MCF-7, PC-12, HUVEC
Primary cells: hMSC, Mouse oocyte stem cell, PBMC (Peripheral Blood Mononuclear cell), Neuronal stem cell
Embryonic cell: Mouse embryonic cell

Does JuLI™ Stage measure cell size?
Yes, we have a software ruler and also a scale bar.

Can the JuLI™ Stage detect bacterial cells?
Yes, we can use the 20x objective lens for bacterial detection.

What kind of objective lens can we use?
JuLI™ Stage offers three different magnifications of the objective lens; 4x, 10x and 20x

How long does it take to capture 96 wells with 1 color?
It takes about 9 minutes.

What is the limit of the detection with 20x objective lens of JuLI™ Stage?
The limit of detection is 1㎛.

Can I measure the counting number of GFP/RFP cells?
Yes, we are providing the viewer software, which you can install on your desk top computer.

How do I optimize the cell migration assay for my cell type?
It depends on the assay medium and cell density, you should attempt to find out the optimal condition of your cells with trial and error.

Can I make parallel measurement of cell confluence using a fluorescent reagent?
Yes, multiplexed measurements of cell confluence can be made using GFP/RFP/DAPI imaging channel.

Is it difficult to use the stitching function in JuLI™ Stage?
It is easy. You can click the stitching toggle button on the option mode to apply stitching while monitoring. Also, you can determine the range of stitching area.

Can I use 100mm culture dishes with JuLI™ Stage?
Yes, JuLI™ Stage is compatible with microplates (6well, 24well, 48well, 96well and 384well), T25 and T75 culture flasks, Petri dishes (35mm, 60mm, 100mm).

Can I use V-Bottom well plate or clear-bottom well plate?
You can expect the better result with clear-bottom well plates. However, we do not recommend to use V-bottom well plates due to the difficulty of focusing objectives.

How come the time-lapse images become dark or bright?
* Please check the following to enhance images.
* Cell culture flask should be wetted by the culture media before making a movie.
* Warm up the instrument for 2 hours before monitoring (The power should be on when warming up the instrument).
* Eliminate any dust on the culture dish.
* Remove any condensation on the lid of the culture dish.
* To prevent any problem such as shaking of culture dish or inflowing of light, take extreme caution when you open or close an incubator door during monitoring.

If I want to change the objective lens while I am monitoring, how can I change it?
You can change the objective lens manually.

Doesn’t it have any problem caused by humidity inside an incubator such as the image is out of focus?
In order to prevent such problem, we strongly recommend the following two things before start of monitoring.
* Warm up the JuLI™ Stage for 2 hours inside the incubator.
* Coat the well plate cover with the cell culture media or the clean PBS.

What are the dimensions of the JuLI™ Stage?
It is 429 (W) X 310 (D) X 324 (H) mm. You can place it inside most incubators in your lab.

What do you chemical recommend to wipe the surface of JuLI™ Stage?
We recommend you to use 70% Ethanol.

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